Ed Pryzdial

Contact InformationEd Pryzdial
Life Sciences Centre
#4365 – 2350 Health Sciences Mall
Vancouver, BC, Canada V6T 1Z3
Tel: 1-604-822-3823
Fax: 1-604-822-7135
Email: ed.pryzdial@blood.ca

Current Positions

  • Scientist, Canadian Blood Services, Centre for Innovation.
  • Clinical Professor, Pathology and Laboratory Medicine.
  • Associate Director, Centre for Blood Research.

Research Interests

  • Blood Proteins.
  • Coagulation and Fibrinolysis.
  • Role of Blood Proteins in Viral Infection.

The general expertise in our laboratory is the function of blood proteins and there novel application. By combining biochemistry and molecular engineering, we have discovered new functions of several proteins with possible therapeutic or diagnostic applications to heart disease and virus infection. Our long-range goal is to understand how these findings may impact on other blood protein functions and thereby contribute to optimizing the value derived from blood constituents and their substitutes.

Communication Between Clot-Forming and Clot-Dissolving Proteins

Clot generation (coagulation) and subsequent clearance (fibrinolysis) are fundamental biological processes. A high degree of communication between these opposing pathways is necessary to ensure that clots form and dissolve sequentially, and only when needed. The biological effector of coagulation, thrombin, is activated by an enzyme complex consisting of the protease factor Xa (FXa) and its cofactor Va (FVa). In recent work, Dr. Pryzdial’s group identified a previously unknown mechanism of communication between these clotting factors and the fibrinolysis pathway. The data revealed that FXa and FVa accelerate the clot-buster, tissue plasminogen activator (tPA), which is used as an important therapeutic. These studies suggest that the current fibrinolysis dogma must be revised to include auxiliary tPA cofactors, such as FXa and FVa, in the vicinity of the clot to further understand vascular health and pathology.

Blood Proteins Exploited by Viruses

For decades many viruses have been linked to heart disease. To understand the molecular basis of the clinical correlation, Dr. Pryzdial’s group found that at least herpes simplex virus type 1 and type 2, and cytomegalovirus can directly assemble clotting protein complexes on their surfaces to generate thrombin. This bypasses the normally strict regulation of coagulation imposed by cells. Virus initiated clot formation not only contributes to heart disease, but enhances the susceptibility of host cell infection through protease activated receptors and possibly annexins. Dr. Pryzdial and his lab members are further dissecting the specific host- and virus-derived proteins on the virus surface that trigger coagulation and their role in virus propagation.

Publications

  1. Carter RLR, Talbot K, Woosuk SH, Meixner, SC,Van Der Gugten JG, Holmes DT, Cote HCF, Kastrup CJ, Smith TW, Lee AYY, Pryzdial ELG. (2018). Rivaroxiban and apixaban induce clotting factor Xa fibrinolytic activity. Journal of Thrombosis and Haemostasis. epub: https://doi.org/10.1111/jth.14281.
  2. Pryzdial, ELG, Lee, F Lin, B Carter, RL Tegegn, TZ Belletruti, M. (2018). Blood Coagulation Dissected. Transfusion and Apheresis Science. 57(4):449-457.
  3. Pryzdial ELG. (2017). Maestro tissue factor reaches new hEIGHT. Blood. 5;130(14):1604-1605.
  4. Edward L. G. Pryzdial, Michael R. Sutherland, Ayo Y. Simon. (2016). Dengue Virus Replication by Platelets. International Society of Blood Transfusion Science Series. 12: 154-160.
  5. Manu Thomas Kalathottukaren, Libin Abraham, Benjamin F.L. Lai, Rajesh A. Shenoi, Federico I. Rosell, Edward L.G. Pryzdial, James H. Morrissey, Charles A. Haynes and Jayachandran N. Kizhakkedathu. (2016). Alteration of clotting and clot morphology by protamine are avoided using the heparin and polyphosphate inhibitor, UHRA. Blood. 0: 1-10. 129(10): 1368–1379.
  6. Michael R. Sutherland, Ayo Y. Simon, Katherine Serrano, Peter Schubert, Jason P. Acker and Edward L.G. Pryzdial. (2016). Dengue virus persists and replicates during storage of platelets and red blood cell units. Transfusion. 56: 1129-37.
  7. Edward L.G. Pryzdial, Scott C. Meixner, Kimberley Talbot, Louise J. Eltringham-Smith, James R. Baylis, Frank M.H. Lee, Christian J. Kastrup, William P. Sheffield. (2016). Thrombolysis by chemically modified coagulation factor Xa. Journal of Thrombosis and Haemostasis. 14(9):1844-54.
  8. Hun Yeon J, Chan KY, Wong TC, Chan K, Sutherland MR, Ismagilov RF, Pryzdial EL, Kastrup CJ. (2015). A biochemical network can control formation of a synthetic material by sensing numerous specific stimuli. Science Reports. 15(5): 10274.
  9. Edward L.G. Pryzdial, Scott C. Meixner, Kimberley Talbot, Louise J. Eltringham-Smith, James R. Baylis, Christian J. Kastrup, William P. Sheffield. (2015). Thrombolysis is facilitated by chemically modified coagulation factor Xa. Journal of Thrombosis and Haemostasis. 14: 1844-54.
  10. Simon AY, Sutherland MR, Pryzdial EL. (2015). Dengue virus binding and replication by platelets. Blood. 126(3): 378-85.
  11. Edward L.G. Pryzdial, Michael R. Sutherland, Wolfram Ruf. (2014). The procoagulant envelope virus surface: Contribution to enhanced infection. Thrombosis Research. 133: S15-S17.
  12. Louise J. Eltringham-Smith, Xi Lei, Adili Reheman, Melissa D. Lambourne, Edward L. Pryzdial, Heyu Ni and William P. Sheffield. (2014). The fibrinogen but not the Factor VIII content of transfused plasma determines its effectiveness at reducing bleeding in coagulopathic mice. Transfusion. 55: 1040-50.
  13. Sheffield WP, Bhakta V, Talbot K, Pryzdial EL, Jenkins C. (2013). Quality of frozen transfusable plasma prepared from whole blood donations in Canada: An update. Transfusion and apheresis science. 49: 440-6.
  14. Talbot K, Meixner SC, Pryzdial EL. (2013). Proteolytic modulation of factor Xa-antithrombin complex enhances fibrinolysis in plasma. Biochimica et Biophysica Acta. 1834: 989-95.
  15. Sutherland MR, Ruf W, Pryzdial EL. (2012). Tissue factor and glycoprotein C on herpes simplex virus type 1 are protease-activated receptor 2 cofactors that enhance infection. Blood. 119(15): 3638-45.
  16. Pryzdial ELG, Lin BH, Sutherland MR. (2015). Virus-Platelet Associations. Gresele P, Lopez JA, Kleiman NS, Page CP. Platelets in Thrombotic and Non-Thrombotic Disorders. Second: 1-12. Submitted, Springer, United States.

Education

  • Ph.D., University of Toronto, Canada, 1987.
  • B.Sc. (Biology, Chemistry, Biochemistry) University of Toronto, Canada, 1981.