Ed Pryzdial

Contact InformationEd Pryzdial
Life Sciences Centre
#4365, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3
Tel 1-604-822-3823
Fax 1-604-822-7135

Current Positions

  • Scientist, Canadian Blood Services
  • Clinical Professor, Pathology and Laboratory Medicine
  • Associate Director, Centre for Blood Research

Research Interests

  • Blood Proteins
  • Coagulation and Fibrinolysis
  • Role of Blood Proteins in Viral Infection

The general expertise in our laboratory is the function of blood proteins and there novel application. By combining biochemistry and molecular engineering, we have discovered new functions of several proteins with possible therapeutic or diagnostic applications to heart disease and virus infection. Our long-range goal is to understand how these findings may impact on other blood protein functions and thereby contribute to optimizing the value derived from blood constituents and their substitutes.

Communication Between Clot-Forming and Clot-Dissolving Proteins

Clot generation (coagulation) and subsequent clearance (fibrinolysis) are fundamental biological processes. A high degree of communication between these opposing pathways is necessary to ensure that clots form and dissolve sequentially, and only when needed. The biological effector of coagulation, thrombin, is activated by an enzyme complex consisting of the protease factor Xa (FXa) and its cofactor Va (FVa). In recent work, Dr. Pryzdial’s group identified a previously unknown mechanism of communication between these clotting factors and the fibrinolysis pathway. The data revealed that FXa and FVa accelerate the clot-buster, tissue plasminogen activator (tPA), which is used as an important therapeutic. These studies suggest that the current fibrinolysis dogma must be revised to include auxiliary tPA cofactors, such as FXa and FVa, in the vicinity of the clot to further understand vascular health and pathology

Blood Proteins Exploited by Viruses

For decades many viruses have been linked to heart disease. To understand the molecular basis of the clinical correlation, Dr. Pryzdial’s group found that at least herpes simplex virus type 1 and type 2, and cytomegalovirus can directly assemble clotting protein complexes on their surfaces to generate thrombin. This bypasses the normally strict regulation of coagulation imposed by cells. Virus initiated clot formation not only contributes to heart disease, but enhances the susceptibility of host cell infection through protease activated receptors and possibly annexins. Dr. Pryzdial and his lab members are further dissecting the specific host- and virus-derived proteins on the virus surface that trigger coagulation and their role in virus propagation.

Selected Publications

  1. Pryzdial ELG. Maestro tissue factor reaches new hEIGHT. Blood. 2017 Oct 5;130(14):1604-1605
  2. Pryzdial EL, Meixner SC, Talbot K, Eltringham-Smith LJ, Baylis JR, Lee FM, Kastrup CJ, Sheffield WP. J Thrombolysis by chemically modified coagulation factor Xa.Thromb Haemost. 2016 Sep;14(9):1844-54.
  3. E. L. G. Pryzdial M. R. Sutherland A. Y. Simon, Dengue virus replication by platelets. 2016 ISBT Science Series Nov; 12, 154-60
  4. Sutherland MR, Simon AY, Serrano K, Schubert P, Acker JP, Pryzdial EL. Dengue virus persists and replicates during storage of platelet and red blood cell units. Transfusion. 2016 May;56(5):1129-37.
  5. Simon AY, Sutherland MR, Pryzdial EL. Dengue virus binding and replication by platelets. Blood. 2015 Jul 16;126(3):378-85.
  6. Pryzdial EL, Sutherland MR, Ruf W. The procoagulant envelope virus surface: contribution to enhanced infection. Thromb Res. 2014 May;133 Suppl 1:S15-7
  7. Talbot K, Meixner SC, Pryzdial EL. Proteolytic modulation of factor Xa-antithrombin complex enhances fibrinolysis in plasma. Biochim Biophys Acta. 2013 Jun;1834(6):989-95.
  8. Vanden Hoek, A.L., Talbot, K., Carter, I.S.R., Vickars, L., Carter, C.J., Jackson, S.C., MacGillivray, R.T.A and Pryzdial, E.L.G. (2012) Coagulation Factor X Arg386 specifically affects activation by the intrinsic pathway: A novel patient mutation. Journal of Thrombosis and Haemostasis Oct 5 doi: 10.111/jth.12021
  9. Sutherland, M.R., Ruf, W. and Pryzdial, E.L.G. (2012) Tissue factor and glycoprotein C on herpes simplex virus type 1 are protease-activated receptor 2 cofactors that enhance infecton. Blood 119:3638-3645.
  10. Krisinger M, Goebeler V, Lu Z, Meixner SC, Myles T, Pryzdial ELG, Conway EM. Revising the terminal complement pathway: Thrombin generates previously unidentified C5 products which augment lytic activity. Blood 2012. 120(8):1717-25.
  11. Gershom. E.S., Vanden Hoek, A.L., Sutherland, M.R. and Pryzdial, E.L.G. (2012) Herpesviruses enhance fibrin clot lysis. Thrombosis and Haemostasis 107:760-768
  12. Carter, S.R., Vanden Hoek, A.L., Pryzdial, E.L.G. and MacGillivray, R.T.M. (2011) Thrombin A-Chain: activation remnant or allosteric effector? Thrombosis doi:10.1155/2010/416167
  13. Martinez-Gutierrez, F., Thi ,E.P., Silverman, J.M., de Oliveira, C., Svensson, S., Vanden Hoek, A., Sanchez,E.M., Reiner, N.E., Gaynor,E., Conway, E.M., Pryzdial, E.L.G., Orrantia, E., Ruiz, F., Av-Gay, Y., Bach, Y. (2012) Antibacterial activity, inflammatory response, coagulation and cytotoxicity effects of silver nanoparticles. Nanoparticles 8:328-336
  14. Talbot, K., Song, J., Hewitt, J., Serrano, K., Ho, M., MacGillivray, R.T.A., Carter, C.J. and Pryzdial, E.L.G. (2010) A novel compensating mechanism for homozygous coagulation factor V deficiency suggested by enhanced activated partial thromboplastin time after reconstitution with normal factor V . British Journal of Haematology, 151:198-200
  15. Gershom, E.S., Sutherland, M.R. and Pryzdial, E.L.G. (2010) Herpes simplex virus initiates the contactphase and intrnsic pathway of coagulation., Journal of Thrombosis and Haemostasis, 8:1037-104
  16. Talbot, K., Meixner, S.C. and Pryzdial, E.L.G. (2010) Enhanced fibrinolysis by proteolysed coagulation factor Xa. Biochimica et Biophysica Acta 1804:723-730
  17. Song, J., Talbot, K., Hewitt, J., MacGillivray, R.T.A. and Pryzdial, E.L.G. (2009) Differential contributions of Glu96, Asp102 and Asp111 to coagulation factor Va metal ion binding and subunit stability. Biochemical Journal 422:257-264


  • Ph.D., University of Toronto, Canada, 1987
  • B.Sc. (Biology, Chemistry, Biochemistry) University of Toronto, Canada, 1981