Juergen Kast

Contact Information
Biomedical Research Centre
#401, 2222 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3
Tel 1-604-822-7814
Fax 1-604-822-7815
kast@brc.ubc.ca
Lab Website: http://brc.ubc.ca/proteomics-and-cell-signaling-jurgen-kast/

Current Positions

  • Associate Professor, Chemistry, Science
  • Associate Professor, Biomedical Research Centre
  • Scientific Director, BC Proteomics Network

Research Interests

The focus of our research continues to be on the development and application of proteomic methods to study signal transduction mechanisms in human cells. This includes the use of global protein expression screens to discover protein markers linked to aspects of human health, and the analysis of the interactions of individual signaling proteins and their dynamic changes in health and disease. It is hoped that this will ultimately lead to the design of improved diagnostic tools and treatments. We are currently working on the following themes:

  • Discovery of novel protein markers
  • Analysis of stable and transient protein interactions
  • Comprehensive detection of post-translational modifications

Publications

  1. Miao Q, Zhang CC, Kast J. Chemical proteomics and its impact on the drug discovery process. Expert Rev Proteomics. 2012 Jun;9(3):281-91.
  2. Klockenbusch C, O’Hara JE, Kast J. Advancing formaldehyde cross-linking towards quantitative proteomic applications. Anal Bioanal Chem. 2012 Sep;404(4):1057-67.
  3. Robb DB, Rogalski JC, Kast J, Blades MW. Liquid chromatography-atmospheric pressure electron capture dissociation mass spectrometry for the structural analysis of peptides and proteins. Anal Chem. 2012 May 1;84(9):4221-6.
  4. Robb DB, Rogalski JC, Kast J, Blades MW. A new ion source and procedures for atmospheric pressure-electron capture dissociation of peptides. J Am Soc Mass Spectrom. 2011 Oct;22(10):1699-706.
  5. Lehnertz B, Rogalski JC, Schulze FM, Yi L, Lin S, Kast J, Rossi FM. p53-dependent transcription and tumor suppression are not affected in Set7/9-deficient mice. Mol Cell. 2011 Aug 19;43(4):673-80.
  6. Kast J. A quick reality check for microRNA target prediction. Expert Rev Proteomics. 2011 Apr;8(2):149-52.
  7. Bouman L, Schlierf A, Lutz AK, Shan J, Deinlein A, Kast J, Galehdar Z, Palmisano V, Patenge N, Berg D, Gasser T, Augustin R, Trümbach D, Irrcher I, Park DS, Wurst W, Kilberg MS, Tatzelt J, Winklhofer KF. Parkin is transcriptionally regulated by ATF4: evidence for an interconnection between mitochondrial stress and ER stress. Cell Death Differ. 2011 May;18(5):769-82.
  8. Zhang CC, Rogalski JC, Evans DM, Klockenbusch C, Beavis RC, Kast J. In silico protein interaction analysis using the global proteome machine database. J Proteome Res. 2011 Feb 4;10(2):656-68.
  9. Robb DB, Rogalski JC, Kast J, Blades MW. Atmospheric pressure-electron capture dissociation of peptides using a modified PhotoSpray ion source. Rapid Commun Mass Spectrom. 2010 Nov 30;24(22):3303-8.
  10. Walsh GM, Rogalski JC, Klockenbusch C, Kast J. Mass spectrometry-based proteomics in biomedical research: emerging technologies and future strategies. Expert Rev Mol Med. 2010 Sep 23;12:e30.
  11. Toews J, Rogalski JC, Kast J. Accessibility governs the relative reactivity of basic residues in formaldehyde-induced protein modifications. Anal Chim Acta. 2010 Aug 31;676(1-2):60-7.
  12. Klockenbusch C, Kast J. Optimization of formaldehyde cross-linking for protein interaction analysis of non-tagged integrin beta1. J Biomed Biotechnol. 2010;2010:927585.
  13. Zhang CC, Kast J. Applications of current proteomics techniques in modern drug design.Curr Comput Aided Drug Des. 2010 Sep;6(3):147-64.
  14. Schubert P, Thon JN, Walsh GM, Chen CH, Moore ED, Devine DV, Kast J. A signaling pathway contributing to platelet storage lesion development: targeting PI3-kinase-dependent Rap1 activation slows storage-induced platelet deterioration. Transfusion. 2009 Sep;49(9):1944-55.
  15. Wong JP, Reboul E, Molday RS, Kast J. A carboxy-terminal affinity tag for the purification and mass spectrometric characterization of integral membrane proteins. J Proteome Res. 2009 May;8(5):2388-96.
  16. Walsh GM, Lin S, Evans DM, Khosrovi-Eghbal A, Beavis RC, Kast J. Implementation of a data repository-driven approach for targeted proteomics experiments by multiple reaction monitoring. J Proteomics. 2009 Jul 21;72(5):838-52.

Education

  • Postdoc, EMBL Heidelberg, 1998-2001
  • Ph.D. University of Konstanz, 1998
  • B.A.Sc. University of Konstanz, 1993

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